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مقاله
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Abstract
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Title:
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Intravitreal propranolol inhibits laser induced choroidal neovascularization in animal model
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Author(s):
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Hamid Ahmadieh, Ramin Nourinia, Mozhgan Rezaei kanavani, Amir Kahrkabodi, Mohsen Khandagi
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Presentation Type:
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Oral
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Subject:
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Posterior Segment and Uveitis
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Others:
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Presenting Author:
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Name:
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Ramin Nourinia
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Affiliation :(optional)
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ophthalmic research center, shahid beheshti university of medical science
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E mail:
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ramin.retin@gmail.com
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Phone:
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04413365316
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Mobile:
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09128249332
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Purpose:
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Our previous study had determined the safe dose of intravitreal propranolol. This study was designed to assess the efficacy of intravitreal propranolol in the management of laser induced choroidal neovascularization (CNV).
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Methods:
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42 Six-week-old female C57BL6J mice were included in this study. Rupture of the Bruch membrane in right eye of all C57BL6J mice was induced by laser. Then the mice were randomized into 2 groups: I) control group (21 animals) who received intravitreal BSS, and II) treatment group (21 animals) that received intravitreal 0.3µg/5µl propranolol. Neovascularization was measured on choroidal-scleral flat mounts using intercellular adhesion molecule 2 immunofluorescence staining.
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Results:
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Four eyes from 4 control mice were phthisic at the time of enucleation and therefore were excluded. Mice eyes treated with intravitreal injection of propranolol showed significantly smaller neovascular choroidal outgrowths compared to the control. An approximately 4.8-fold decrease in the average CNV area in each eye was observed in the propranolol-treated (mean [SE], 17945 [25971] µm2; n=21 eyes) compared with the control (mean [SE], 86821 [49274] µm2; n=17 eyes), which was statistically significant (p<0.001).
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Conclusion:
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This study demonstrated that intravitreal injection of propranolol could be effective in the treatment of CNV.
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Attachment:
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