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مقاله
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Abstract
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Title:
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The molecular method in diagnosis of ocular toxoplasmosis in some major ophthalmic centers in Tehran
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Author(s):
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Seyyed Javad Seyyed Tabaei*1, Seyed Ali Tabatabaei2, Vafa Saber1, Ali Haghighi1, Yadollah Mehrabi3
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Presentation Type:
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Poster
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Subject:
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Posterior Segment and Uveitis
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Others:
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Presenting Author:
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Name:
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Vafa Saber
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Affiliation :(optional)
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1Parasitology and Mycology department, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2Farabi Hospital, Tehran University of Medical Sciences, Tehran, Iran 3Epidemiolo
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E mail:
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vafa.saber125@gmail.com
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Phone:
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09124494580
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Mobile:
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09124494580
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Purpose:
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Toxoplasma gondii is an opportunistic infection. The parasite causes chorioretinitis. The posterior pole scares persist a serious threat. Other symptoms consist of blurred vision, photophobia, vitreous inflammation etc. Molecular methods are complementary for recognition. The aim is to evaluate efficiency of molecular method in isolation of T. gondii.
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Methods:
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Blood sampling had done. For examining possible relapse, re-sampling was done in 15 and 30 days. Thereafter, the buffy coat was employed for DNA extraction. For performing the PCR, primer of the gene B1was applied.
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Results:
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From the 71 samples, 34 patients (47%) had positive form of the disease. However, evaluating the first relapse, two weeks after the first step of the disease, among the 41 patients, 29 (70%) had positive reaction of the disease. These patients showed positive PCR in the first step. In the third step (30 days later), from 19 patients, 9 (47%) had positive reaction. In the second and the third steps there were no incidents.
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Conclusion:
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The diagnosis relays on clinical tests. Clinical symptoms or differentiation of the disease from alternative symptoms make the diagnosis problematic. However, sensitivity and characteristic of molecular PCR is of interest. For definite diagnosis, clinical and complementary methods such as PCR can be employed.
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Attachment:
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7010.pdf
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