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مقاله
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Abstract
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Title:
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OTX2 positive retinal progenitor cells derived from neurosphere offshoot from bone marrow stromal stem cell
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Author(s):
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Hamid Aboutaleb kadkhodaeian 1,2, Taki Tiraihi,*1, Hamid Ahmadieh3, Hosein ziaii Ardakani 1,3, Narsis Daftarian3.
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Presentation Type:
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Poster
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Subject:
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Posterior Segment and Uveitis
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Others:
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Presenting Author:
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Name:
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Hamid Aboutaleb Kadkhodaeean
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Affiliation :(optional)
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1Shefa neuroscience research center ,Khatam-Al-Anbia Hospital, Tehran, Iran
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E mail:
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habootaleb92@gmail.com
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Phone:
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Mobile:
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09377711768
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Purpose:
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: In the mature retina, Otx2 is expressed in three cell types: bipolar and photoreceptor cells that belong to the neural retina and retinal pigment epithelium (RPE), a neighbour structure that forms a tightly interdependent functional unit together with photoreceptor cells.
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Methods:
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we isolated the bone marrow stromal stem cells from pigmented rats and after reaching to passage 3-5, differentiated to neurosphere. The neurosphere medium changed to differentiated medium and in floating manner specific marker for progenitor cells (OTX2) and photoreceptor cell investigated.
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Results:
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our results showed that differentiated neurosphere 7-14 days after induction, express the retinal progenitor OTX2 marker and also Rhodopsin and recoverin, specific markers for photoreceptor cells.
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Conclusion:
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for the first time in vitro generation of OTX2 positive retinal progenitor cells create the hopeful for planning the cell replacement therapy for AMD and RP.
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Attachment:
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